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A glucose biofuel cell on a flexible bacterial nanocellulose film was prepared. The bioelectrodes were printed using gold ink as the conductive material. The anode was modified with colloidal platinum for the oxidation of glucose. The cathode was modified with a nanocomposite comprising gold nanoparticles (AuNPs) and silver oxide (Ag2O) nanoparticles. The cathode was characterized via cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and UV spectroscopy techniques. The assembled biofuel cell generated a maximum open circuit voltage (V oc ) of 0.485 V, short circuit current (I sc ) of 0.352 mA/cm 2 , and a maximum peak power density (P max ) of 0.032 mW/cm 2 when operating in 30 mM concentration. This system showed a stable and linear performance with a linear range of 1 mM to 30 mM glucose. The gold printed electrode process is applicable to the development of wearable and implantable abiotic biofuel cell.